Salud Capital
Salud Capital Research · April 2025
Timely Research · April 2025

DeFi and Blockchain in April 2025: AI Agents Are Starting to Run the Protocols

Something structural has shifted in DeFi. Autonomous AI agents are beginning to handle tasks — liquidity management, yield optimization, risk parameter adjustment — that human traders and risk teams used to own. The on-chain agent economy is nascent but moving faster than anyone predicted six months ago. Here is what is actually live, what is coming, and how to think about the investment implications.

DeFiOn-Chain AgentsGauntletAaveUniswap v4ElizaOSERC-4337ai16z
✎ Salud Capital Research📅 April 2025⚠ April 2025 snapshot
$250K+
Daily On-Chain Agents
Early 2025, 400% YoY
$25M
ai16z DAO AUM
AI-managed portfolio
ERC-4337
Agent Wallets Live
Session key delegation
68%
New DeFi w/ AI
Q1 2025 protocols
$4B
AI Agent Market
2025 on-chain segment
01 · The Structural Shift

AI Agents Are Becoming the New Primary Users of DeFi

DeFi was built assuming human users would interact with protocols in real time. That assumption is rapidly becoming obsolete. In the first quarter of 2025, more than 68% of new DeFi protocols launched included at least one AI agent for trading or liquidity management. Daily active on-chain AI agents have crossed 250,000, representing 400% year-over-year growth. The protocol is now more likely to be queried by an agent than by a human directly.

The shift is driven by a convergence of three developments that came together faster than most anticipated. First, the frontier LLMs became capable enough to reliably execute multi-step tool calls without frequent hallucination failures. Second, ERC-4337 account abstraction created a wallet architecture where agents can hold session keys with defined spending limits — making it safe to delegate transaction authority to an agent without handing over the master private key. Third, open-source agent frameworks (ElizaOS, LangGraph) lowered the engineering barrier for on-chain agent deployment from months to weeks.

The ai16z DAO as proof of concept: The ai16z DAO — now ElizaOS — has been running autonomous AI agents managing a $25 million crypto portfolio since October 2024. The agents analyze market conditions, evaluate investment proposals from community members through a “Marketplace of Trust” credibility scoring system, and execute trades on-chain without human intervention between decisions. Six months of live data is the strongest possible evidence that the “agent as economic actor” model is not theoretical.
02 · What Is Actually Live

The On-Chain AI Applications That Work Right Now

ApplicationWhat the Agent DoesWho Is Doing ItMaturity
Risk Parameter Optimization Simulation-driven stress testing of collateral ratios, interest rate curves, and liquidation thresholds; real-time parameter adjustment based on market conditions Gauntlet, Chaos Labs (for Aave, Compound, Morpho) Production
Autonomous Portfolio Management AI fund managers analyzing on-chain data, executing trades, managing positions without human intervention between decisions ai16z DAO / ElizaOS ($25M AUM) Production
Concentrated Liquidity Management Dynamic range adjustment for concentrated LP positions on Uniswap v3/v4; fee tier optimization; auto-compounding Sommelier, Beefy AI strategies Production
Smart Contract Auditing LLM-powered vulnerability scanning on new protocol deployments; CI/CD security integration for audit-before-deploy ChainGPT Solidity LLM, Slither + AI Maturing
Cross-Chain Intent Execution Natural-language instruction → optimal cross-chain routing → execution via ERC-4337 session keys Solana Agent Kit, LangGraph flows Maturing
DAO Governance Delegation Agents analyze proposals against predefined investment thesis, cast votes, draft governance commentary on X Autonolas governance agents Maturing
03 · Infrastructure Layer

The Stack That Makes On-Chain Agents Possible

Understanding the on-chain agent economy as an investor requires mapping the infrastructure stack. Each layer has distinct protocols competing for dominance, distinct token economics, and distinct risk profiles. The five-layer stack below is the current standard:

LayerFunctionLeading Protocols (Apr 2025)
ComputeGPU supply for AI training and inference — the decentralized cloud layerRender (RENDER), Akash (AKT), io.net
IntelligenceDecentralized model training, validation, inference marketplaceBittensor (TAO), ChainGPT
Agent OSFramework for building agents with persistent identity, memory, and cross-chain capabilitiesElizaOS, Autonolas
Wallet / ExecutionAgent wallet delegation, session keys, spend limits, transaction executionERC-4337, Safe{Wallet}, Privy
Data / OraclesOn-chain data feeds, indexing, and verifiable off-chain data for agent decision-makingThe Graph (GRT), Chainlink
The connectedness thesis applied to DeFi AI: The investment opportunity here is in the infrastructure that every agent must use, not individual agent products. Render and Akash benefit from every new agent deployment. Bittensor benefits from every inference query sent to decentralized networks. The Graph benefits from every agent that queries on-chain data. These are toll roads, and traffic is growing 400% annually.

References

R-01
Autonomous Agents on Blockchains: Standards, Execution Models, and Trust Boundaries
arXiv:2601.04583 · January 2026 (research period through early 2025) · Comprehensive academic survey; 20 production systems analyzed; taxonomy of integration patterns
arxiv.org/abs/2601.04583
R-02
ERC-4337: Account Abstraction Standard
Ethereum Foundation · Vitalik Buterin et al. · Smart account architecture; session key delegation enabling agent wallet authority
eips.ethereum.org/EIPS/eip-4337
R-03
Gauntlet — Risk Parameter Optimization for DeFi
Gauntlet · Simulation-driven collateral and rate parameter management for Aave, Compound, and Morpho; live production risk system
gauntlet.xyz
R-04
Blockchain App Factory — AI Agents Development 2026 Technical Guide
Blockchain App Factory · March 2026 · 250K+ daily active agent data; 68% new DeFi adoption statistic; production architecture reference
blockchainappfactory.com/blog/ai-agents-development-2026
R-05
Solana Agent Kit — Technical Documentation (SendAI)
SendAI · 2024–2025 · 50+ Solana on-chain actions for AI agents; DeFi/NFT abstraction; LangChain/ElizaOS integration
github.com/sendaifun/solana-agent-kit